Reporter

Part:BBa_J31011:Design

Designed by: Karmella Haynes   Group: iGEM06_Davidson   (2006-10-29)


RBS-RFP reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 119
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primers were designed to add PstI-NotI-SpeI to the left and XbaI-NotI-EcoRI to the right (reversal of standard BioBrick cloning sites) so that this part could be cloned in the reverse orientation. Placement of a promoter (reverse) to the right of this part should convey RFP expression.


Source

BBa_I13507

References